Use this url to cite publication: https://hdl.handle.net/20.500.12259/60719
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The response of Helianthus annuus to pre-sowing seed treatment with stressors: induced changes in germination, seedling growth and proteome
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
LT | ||
LT | ||
LT | ||
Baniulis, Danas | Lietuvos agrarinių ir miškų mokslų centro filialas Sodininkystės ir daržininkystės institutas | LT |
Filatova, Irina | Institute of Physics of the National Academy of Sciences of Belarus, Belarus | BY |
Lyushkevich, Veronika | Institute of Physics of the National Academy of Sciences of Belarus, Belarus | BY |
Title
The response of Helianthus annuus to pre-sowing seed treatment with stressors: induced changes in germination, seedling growth and proteome
Is part of
Smart Bio : ICSB 2nd international conference, 3-5 May 2018, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2018
Date Issued
Date Issued |
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2018 |
Publisher
Kaunas : Vytautas Magnus University, 2018
Extent
p. 273-273
Field of Science
Abstract
Seed treatment with cold plasma (CP) or electromagnetic field (EMF) is a modern eco-agricultural technology for stimulation of plant germination and performance. Numerousstudies demonstrated the effectiveness of such treatments for enhancing germination andgrowth of a large variety of crops, however the molecular basis of seed response totreatments remain elusive. In order to gain an insight into the molecular mechanisms underlying effect of stressors on plant seeds, we estimated changes induced in differential protein expression of the model plant – sunflower (Helianthus annuus). We studied the effects of pre-sowing seedtreatment, using vacuum (7 min), radio-frequency EMF (5-15 min) and CP (2-7 min), ongermination and growth of the confectionary cultivar ʽNyķrségi feketeʽ. The germination tests were performed both in vitro and in substrate and the obtained results indicated thatthe treatments with CP and EMF had no effect on the proportion of germinated seeds butCP (7 min) and EMF (10 and 15 min) treatments increased both germination rate (by 20-24%) and uniformity of germination (up to 130%). The treatment had effect on morphologyof seedlings that developed from the CP (7 min) and EMF (15 min) treated seeds resultingin reduced height/weight of stems and increased weight of leaves, respectively. Differential protein expression in the leaves of sunflower seedlings was assessed using 2Dgel electrophoresis. Among the 104 differentially expressed proteoforms significantlyhigher abundance of 49 and 38 proteoforms and reduced abundance of 26 and 33proteoforms was detected for the CP and EMF treated experimental groups, respectively.Hierarchical clustering revealed 3 major protein expression groups: two of the groupsincluded majority of the proteoforms that had similarly increased or reduced abundanceafter the CP or EMF treatment.[...]
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)