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Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Aleknavičiūtė, Vesta;Paužaitė, Giedrė;Mildažienė, Vida;Baniulis, Danas;Filatova, Irina;Lyushkevich, Veronika
Title: The response of Helianthus annuus to pre-sowing seed treatment with stressors: induced changes in germination, seedling growth and proteome
Is part of: Smart Bio : ICSB 2nd international conference, 3-5 May 2018, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2018
Extent: p. 273-273
Date: 2018
Keywords: Cold plasma;Helianthus annuus;Seed treatment
ISBN: 9786098104486
Abstract: Seed treatment with cold plasma (CP) or electromagnetic field (EMF) is a modern eco-agricultural technology for stimulation of plant germination and performance. Numerousstudies demonstrated the effectiveness of such treatments for enhancing germination andgrowth of a large variety of crops, however the molecular basis of seed response totreatments remain elusive. In order to gain an insight into the molecular mechanisms underlying effect of stressors on plant seeds, we estimated changes induced in differential protein expression of the model plant – sunflower (Helianthus annuus). We studied the effects of pre-sowing seedtreatment, using vacuum (7 min), radio-frequency EMF (5-15 min) and CP (2-7 min), ongermination and growth of the confectionary cultivar ʽNyķrségi feketeʽ. The germination tests were performed both in vitro and in substrate and the obtained results indicated thatthe treatments with CP and EMF had no effect on the proportion of germinated seeds butCP (7 min) and EMF (10 and 15 min) treatments increased both germination rate (by 20-24%) and uniformity of germination (up to 130%). The treatment had effect on morphologyof seedlings that developed from the CP (7 min) and EMF (15 min) treated seeds resultingin reduced height/weight of stems and increased weight of leaves, respectively. Differential protein expression in the leaves of sunflower seedlings was assessed using 2Dgel electrophoresis. Among the 104 differentially expressed proteoforms significantlyhigher abundance of 49 and 38 proteoforms and reduced abundance of 26 and 33proteoforms was detected for the CP and EMF treated experimental groups, respectively.Hierarchical clustering revealed 3 major protein expression groups: two of the groupsincluded majority of the proteoforms that had similarly increased or reduced abundanceafter the CP or EMF treatment.[...]
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Lietuvos agrarinių ir miškų mokslų centro filialas Sodininkystės ir daržininkystės institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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