Use this url to cite publication: https://hdl.handle.net/20.500.12259/93061
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Investigation of the genetic origin of monument - tree The Witch Tree (Picea abies) In Rambynas Regional Park
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Title
Investigation of the genetic origin of monument - tree The Witch Tree (Picea abies) In Rambynas Regional Park
Is part of
Smart Bio : ICSB 3rd international conference, 02-04 May 2019, Kaunas, Lithuania : abstract book / Vytautas Magnus university. Panevėžys : UAB "Reklamos forma", 2019, Nr. 3
Date Issued
Date Issued |
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2019 |
Publisher
Panevėžys : UAB "Reklamos forma"
Extent
p. 167-167
Field of Science
Abstract
The Witch tree consists of several different genotypes (seedlings) created by debarking them and binding together so allowing the stems to fuse the cambiums info a single stem at a later age. About the Witch tree, it is a ca. 200 years old Norway spruce tree distinguishing by the large number of mature stems (˃12); located in western Lithuania, near Šilutė in Rambynas Regional Park. We sampled wood for DNA extraction from each the 12 stems of Witch tree and used 2 trees of Picea abies from Bubliškes park as controls. The research was carried out at Vytautas Magnus university Agriculture academy laboratory of Forest genetics. 10 polymorphic microsatellite DNA loci were used to assess the genetic identity of each of the 12 stems of the Witch tree. The alleles were scored on capillary electrophoresis with ABI310. The DNA test showed that the 12 stems possess identical alleles at all 10 loci. This indicates that all 12 stems of the Witch tree belong to the same genotype, individual tree.
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)