Use this url to cite publication: https://hdl.handle.net/20.500.12259/58359
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Electrochemotherapy effectiveness in Hepatoma model in vitro: Hypoxia vs Normoxia
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
Electrochemotherapy effectiveness in Hepatoma model in vitro: Hypoxia vs Normoxia
Is part of
COINS 2018: 13th international conference of life sciences, 28 February – 2 March 2018: book of abstracts. Vilnius: Vilniaus universitetas, Gyvybės mokslų centras, 2018
Date Issued
Date Issued |
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2018 |
Publisher
Vilnius: Vilniaus universitetas, Gyvybės mokslų centras, 2018
Extent
p. 149-151
Field of Science
Abstract
Liver cancer is one of the most common tumors, traditional invasive treatment still faces ineffectiveness or inapplicable. Relatively new approach – combination of cytotoxic compound and external electrical fields - electrochemotherapy (ECT) could get better disease control. ECT application in vivo, revealed lower effectiveness then expected, we hypothesized - extracellular oxygen concentration could be partly responsible. Especially, when physiological aspects (tumor anatomy/location, blood supply et etc.) and ECT specific events (vasoconstriction, oxygen activated bleomycin et ect.) are present. Aim of the study – evaluate effectiveness of ECT in different oxygen environments. Materials and methods: Traditionally cultivated mouse hepatoma MH-22A cells was used as model. Detached cell and cytotoxic, membrane impenetrable agent - bleomycin was used for suspension preparation. Prepared cells were permeabilized by applying single, 2kV/cm external electrical field pulse, for 100μs (electroporated), diluted and seeded in petri dish for colonyforming assay. Prepared petri dishes were placed in incubators in 21% oxygen level (atmospheric/normoxia) or 0,2% oxygen level (hypoxia) immediately after manipulation. Effect of hypoxia in compare to normoxia was evaluated in cases of classical chemotherapy (without electroporation) and ECT using different bleomycin concentration. Results showed, that in case of chemotherapy low bleomycin concentration couldn’t penetrate plasma membrane leading to cancer cell high survivability in normoxia. Cell death was reached only when tremendous bleomycin concentrations (range of mIU) were used. In case of hypoxia, MH-22A cell exhibited even higher resistance to bleomycin, LD50 could not be reached in investigated range. We believe that, design of experiment, and fast ECT effect, limited to distinguish differences between normoxia and hypoxia in ECT, leading to overlapping of cell viability curves.
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)