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Type of publication: Straipsnis konferencijos medžiagoje Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or Scopus DB conference proceedings (P1a)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Jackevičius, Vaidas;Mickevičius, Saulius;Adlienė, Diana;Šatkauskas, Saulius;Paukštytė, Severina
Title: Determination of irradiated cell survival with clonogenic assay
Is part of: Medical physics in the Baltic states : proceedings of the 12th international conference on medical physics, Kaunas, Lithuania, 5-7 October, 2015. Kaunas : Kaunas University of Technology, 2015, nr. 12
Extent: p. 100-103
Date: 2015
Keywords: Clonogenic assay;Survival curve;Cell survival;Radiation
Abstract: The clonogenic cell survival assay determines the ability of a cell to proliferate indefinetely, thereby retaining its reproductive ability to form a large colony or a clone. The colony is defined to consist of at least 50 cells. Clonogenic assay is the method of choise to determinate cell reproductive death after treatment with ionizing radiation. After treatment, cells are incubated for 6 days to form colonies. Colonies are fixed with 96% ethanol, stained with crystal violet and counted using Imade J software. For the analysis of relationship between the radiation dose and the fraction of cells retaining their ability to reproduce, cell survival curves are used
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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