Use this url to cite publication: https://hdl.handle.net/20.500.12259/52585
Determination of irradiated cell survival with clonogenic assay
Type of publication
Straipsnis konferencijos medžiagoje Web of Science duomenų bazėje / Article in conference proceedings in Web of Science database (P1a1)
Author(s)
| Author | Affiliation | |
|---|---|---|
Jackevičius, Vaidas | ||
Title
Determination of irradiated cell survival with clonogenic assay
Is part of
Medical physics in the Baltic states : proceedings of the 12th international conference on medical physics, Kaunas, Lithuania, 5-7 October, 2015. Kaunas : Kaunas University of Technology, 2015, nr. 12
Date Issued
| Date |
|---|
2015 |
Publisher
Kaunas : Kaunas University of Technology
Is Referenced by
Extent
p. 100-103
Abstract (en)
The clonogenic cell survival assay determines the ability of a cell to proliferate indefinetely, thereby retaining its reproductive ability to form a large colony or a clone. The colony is defined to consist of at least 50 cells. Clonogenic assay is the method of choise to determinate cell reproductive death after treatment with ionizing radiation. After treatment, cells are incubated for 6 days to form colonies. Colonies are fixed with 96% ethanol, stained with crystal violet and counted using Imade J software. For the analysis of relationship between the radiation dose and the fraction of cells retaining their ability to reproduce, cell survival curves are used.
Type of document
type::text::journal::journal article::research article
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)
ISSN (of the container)
1822-5721
WOS
WOS:000378446500024
Other Identifier(s)
VDU02-000018791