Use this url to cite publication: https://hdl.handle.net/20.500.12259/49340
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Genetic diversity Apodemus sylvaticus and Apodemus flavicollis in Lithuania
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Author(s)
LT | ||
LT | ||
Gedminas, Vaclovas | Tado Ivanausko zoologijos muziejus | LT |
Title
Genetic diversity Apodemus sylvaticus and Apodemus flavicollis in Lithuania
Is part of
The vital nature sign [elektroninis išteklius] : 7-th international scientific conference : abstract book. Kaunas : Vytautas Magnus university, 2013, [no. 7]
Date Issued
Date Issued |
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2013 |
Publisher
Kaunas : Vytautas Magnus university
Extent
p. 42-42
Field of Science
Abstract
Mice of genus Apodemus are wide spred in Europe, western Asia and North Africa. Twenty one species are now recognized, but morfological similarity makes difficulties to recognize these species. Therer is a requirement of molecular markers to clear species identification. The aim of our investigation was to evalute genetic diversity of A. sylvaticus and A. flavicollis in Lithuania using ISSR markers. For DNA research was captured 45 mices from Kaunas district, 13 from Zemaitija National park, 130 rodents from Neringa, 13 from Kaisiadoriai district, and 49 from Juodkrante. Also for research using other mouse from previos Vytautas Magnus University Natural science falculty expeditions from Norway, where were captured 15 Apodemus genus mouse. Using chloroform method „Genomic DNA Purification Kit“(Fermentas, K0512). DNA was extracted from ears and muscules for genome studies. Chosen primers: AP-1 (5‘-(CAG)5GC-3‘), AP-2 (5‘-(CAG)4AC-3’), AP-5 (5‘-(G)6GC-3’), E8S(5‘-TAAATGGGACAGGTAGGACC-3’) and Ep8 1 (5‘CCTTACTGCCTCTTGCTTC-3’). For PCR data analysis used horizontal 1,5% agarose gels. The size of the fragments ranged from 300 to 1700 bp. The primers AP-2, AP-5 and E8S were the most informative to study genetic diversyti of genus Apodemus.
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)