Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/108082
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biologija / Biology (N010);Biofizika / Biophysics (N011)
Author(s): Navickaitė, Diana;Ruzgys, Paulius;Novickij, Vitalij;Jakutavičiūtė, Milda;Maciulevičius, Martynas;Sincevičiūtė, Rūta;Šatkauskas, Saulius
Title: Extracellular-Ca2+-iduced decrease in small molecule electrotransfer efficiency: comparison between microsecond and nanosecond electric pulses
Is part of: Pharmaceutics. Basel : MDPI, 2020, vol. 12, iss. 5
Extent: p. 1-17
Date: 2020
Keywords: Calcium electroporation;Calcium;Microsecond electroporation;Nanosecond electroporation;Membrane repair;Pore resealing
Abstract: Electroporation—a transient electric-field-induced increase in cell membrane permeability—can be used to facilitate the delivery of anticancer drugs for antitumour electrochemotherapy. In recent years, Ca2+ electroporation has emerged as an alternative modality to electrochemotherapy. The antitumor effect of calcium electroporation is achieved as a result of the introduction of supraphysiological calcium doses. However, calcium is also known to play a key role in membrane resealing, potentially altering the pore dynamics and molecular delivery during electroporation. To elucidate the role of calcium for the electrotransfer of small charged molecule into cell we have performed experiments using nano- and micro-second electric pulses. The results demonstrate that extracellular calcium ions inhibit the electrotransfer of small charged molecules. Experiments revealed that this effect is related to an increased rate of membrane resealing. We also employed mathematical modelling methods in order to explain the differences between the CaCl2 effects after the application of nano- and micro-second duration electric pulses. Simulation showed that these differences occur due to the changes in transmembrane voltage generation in response to the increase in specific conductivity when CaCl2 concentration is increased
Internet: https://doi.org/10.3390/pharmaceutics12050422
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vilniaus Gedimino technikos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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