Imunotechnologija

Dalyko anotacija lietuvių kalba

Imunotechnologijos objektas, metodologijos principai, praktinio taikymo reikšmė. Klasikinės ir pažangios technologijos. Imunocheminių ir imunotechnologinių metodų taikymas mokslinių tyrimų laboratorijose biotechnologinės, biofarmacinės pramonės įmonėse, medicininėse diagnostinėse laboratorijose.

Dalyko anotacija užsienio kalba

Object of immunotechnology, principles of methodology, significance of practical application. Classic and advanced technologies. Application of imunotechnological and immunochemical methods in research laboratories in biotechnology, biopharmaceutical industry, medical diagnostic laboratories.

Būtinas pasirengimas dalyko studijoms

Molecular Biology BIO 5001, Molecular Biotechnology BIO 5005

Dalyko turinys

1. Introduction. Object of immunotechnology, technological and practical implications of immunology.
2. Immunoglobulins: types, structure and functions. Production and secretion of immunoglobulins. Basics of immunochemical interactions between antibodies and antigens. Affinity and avidity of antibodies.
3. Production of polyclonal antibodies. Preparation of antigen, immunization, adjuvants, experimental animals. Determination of antibody titer and specificity. Alternative sources of polyclonal antibodies. Purification of polyclonal antibodies.
1. Production of monoclonal antibodies, hybridoma technology, biological models, bioreactors. Monoclonal antibody purification. Chromatographic methods.
2. Genes of immunoglobulins. Recombinant antibodies development technologies. Bispecific, bifunctional antibodies. The antibody library construction and screening. Phage display. Therapeutic antibodies, human monoclonal antibodies.
3. Immunochemical methods: agglutination, immunoprecipitation, immunodiffusion, immunoelectrophoresis.
4. Radioimmunoassay (RIA) for antibody and antigen detection, principles and modifications of method.
5. Enzyme-linked immunosorbent assay (ELISA) for antibody and antigen detection, principles and modifications of method. Direct, indirect, sandwich, competitive ELISA. Typical ELISA protocols, evaluation of the results. Antibody array (chip) technology.
6. Western blot (protein immunoblot). Dot blot.
7. Immunosorbent assay for cell research. Cell ELISA. Enzyme-linked ImmunoSpot (ELISPOT).
8. Light, fluorescence and electron microscopy. Application in immunochemical methods. Immunohistofluorescence. Surface plasmon resonance.
9. Flow cytometry. Method principles, features and application areas. Cell surface and intracellular antigens determination, cell functional characteristics, proliferation, apoptosis, phagocytosis, transfection effectiveness investigation, DNA detection and other methods. Application of flow cytometry for soluble analytes determination. Flow cytometry modifications.
10. Detecting, isolation and fractionating immunocompetent cells. Stimulation of lymphocyte proliferation. Lymphocytes specificity and function determination. Immunity research in vivo.
11. Immunodiagnostics in medical laboratories. Serological HLA typing. Immunosensors - principles and applications to clinical chemistry.
12. Immunotherapy. Vaccines: types, principles of action. Immunocompetent cells (dendritic cell etc.) vaccines. Cytokines as biopharmaceuticals. Generic cytokines. Application of cytokines.

Dalyko studijos valandomis

Lectures – 45 hours, Laboratory Work – 15 hours, Individual Work – 90 hours, Assessments – 10 hours. Total: 160 hours.

Studijų rezultatų vertinimas

Final assessment sums the assessments of written mid-term examination (20%), assessment of laboratory works (30%), and written final examination (50%).

Literatūra

1. 2015 V. Tamošiūnas, I. Pumputienė, R. Kvietkauskaitė. Imunologijos ir imunotechnologijos pagrindai.
ISBN 978-609-467-105-0 e-copy
2. 2012 Abbas A.K., Lichtman A.H., Pillai Sh. Cellular and Molecular Immunology. 8th Edition, 545 pp. Elsevier, W B Saunders Co
3. 2016 Murphy K.P. Janeway's immunobiology. 9th Edition, 869 pp.
Garland Science, Taylor & Francis Group
4. 2013 Kindt T.J., Osborne B. A., Goldsby R.A. Kuby Immunology. 7rd Edition. W H Freeman & Co (Sd)
Supplementary materials
1. 2012 Antibody Engineering. Methods and Protocols, Second Edition, Editors: Chames, Patrick Springer, Humana Press
ISBN 978-1-61779-974-7
2. 2005 Howard M. Shapiro. Practical Flow Cytometry John Wiley & Sons, Inc.
Online ISBN: 9780471722731
3. 2008 J. Gosling, A. Moran. Immunotechnology.
Principles, Concepts and Applications John Wiley & Sons.
ISBN 10: 0471899119
4. 2010 B. Annadurai. A Textbook Of Immunology & Immunotechnology S Chand & Co Ltd
ISBN 10: 8121928079
5. Current Protocols in Immunology Wiley Online Library
Online ISBN: 9780471142737
http://onlinelibrary.wiley.com/book/10.1002/0471142735