Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/99667
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dc.contributor.authorMažeikienė, Ingrida-
dc.contributor.authorKviklys, Darius-
dc.contributor.authorŠikšnianienė, Jūratė Bronė-
dc.contributor.authorZinkus, Dainius-
dc.contributor.authorStanys, Vidmantas-
dc.coverage.spatialLV-
dc.date.accessioned2019-08-01T19:44:01Z-
dc.date.available2019-08-01T19:44:01Z-
dc.date.issued2019-
dc.identifier.issn1407009X-
dc.identifier.otherVDU02-000060082-
dc.identifier.otherScopus : 2-s2.0-85067434798-
dc.identifier.urihttps://content.sciendo.com/view/journals/prolas/73/3/article-p238.xml-
dc.identifier.urihttps://hdl.handle.net/20.500.12259/99667-
dc.identifier.urihttps://doi.org/10.2478/prolas-2019-0038-
dc.description.abstractPrunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. The aim of the study was to obtain virus-free planting material of Prunus domestica L. by chemotherapy in vitro. Ribavirin at concentrations of 10 to 50 mg·l-1 was added to Murashige-Skoog (MS) nutrition medium for virus eradication from microshoots. After a two-week period of chemotherapy, meristems were subcultured monthly on MS medium and proliferation index of shoots was estimated. Microshoots were retested by reverse transcription polymerase chain reaction for presence of virus. At lowest concentrations of 10 mg·l-1 ribavirin was entirely ineffective for ACLSV and 10 to 30 mg·l-1 was ineffective for PNRSV elimination. Ribavirin concentrations of 40 and 50 mg·l-1 destroyed both pathogens. However, at higher concentrations of 40 and 50 mg·l-1 ribavirin exhibited some signs of phytotoxicity on microshoots in the first sub-cultivation period. In order to test the genetic stability of the microplants after chemotherapy the amplified fragment length polymorphism (AFLP) method was applied. Plant genome stability in 'Magna Glauca' at concentrations of 40 mg·l-1 was damaged, as the presence of polymorphic AFLP markers were observeden
dc.description.sponsorshipLietuvos agrarinių ir miškų mokslų centro Sodininkystės ir daržininkystės institutas-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.description.sponsorshipŽemės ūkio akademija-
dc.format.extentp. 238-243-
dc.language.isoen-
dc.relation.ispartofProceedings of the Latvian Academy of Sciences. Section B. Natural, exact, and applied sciences. Riga : Latvian Academy of Science, Vol. 73, no. 3 (2019)-
dc.relation.isreferencedbyBIOSIS Previews-
dc.relation.isreferencedbyZoological Record-
dc.relation.isreferencedbyScopus-
dc.relation.isreferencedbyDe Gruyter-
dc.subjectACLSVen
dc.subjectChemotherapyen
dc.subjectPNRSVen
dc.subjectVirus free planten
dc.subject.otherBiologija / Biology (N010)-
dc.titleInfluence of ribavirin on Prunus domestica L. regeneration, genome stability and virus eradication in vitroen
dc.typeStraipsnis kitose duomenų bazėse / Article in other databases (S4)-
dc.identifier.doihttps://doi.org/10.2478/prolas-2019-0038-
dcterms.bibliographicCitation46-
dc.date.updated2020-06-18T09:17Z-
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item.fulltextWith Fulltext-
item.grantfulltextopen-
crisitem.author.deptHumanitarinių mokslų fakultetas-
crisitem.author.deptHumanitarinių mokslų fakultetas-
crisitem.author.deptHumanitarinių mokslų fakultetas-
crisitem.author.deptHumanitarinių mokslų fakultetas-
crisitem.author.deptAgronomijos fakultetas-
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications
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