Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/99667
Type of publication: Straipsnis kitose duomenų bazėse / Article in other databases (S4)
Field of Science: Biologija / Biology (N010)
Author(s): Mažeikienė, Ingrida;Kviklys, Darius;Šikšnianienė, Jūratė Bronė;Zinkus, Dainius;Stanys, Vidmantas
Title: Influence of ribavirin on Prunus domestica L. regeneration, genome stability and virus eradication in vitro
Is part of: Proceedings of the Latvian Academy of Sciences. Section B. Natural, exact, and applied sciences. Riga : Latvian Academy of Science, Vol. 73, no. 3 (2019)
Extent: p. 238-243
Date: 2019
Keywords: ACLSV;Chemotherapy;PNRSV;Virus free plant
Abstract: Prunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. The aim of the study was to obtain virus-free planting material of Prunus domestica L. by chemotherapy in vitro. Ribavirin at concentrations of 10 to 50 mg·l-1 was added to Murashige-Skoog (MS) nutrition medium for virus eradication from microshoots. After a two-week period of chemotherapy, meristems were subcultured monthly on MS medium and proliferation index of shoots was estimated. Microshoots were retested by reverse transcription polymerase chain reaction for presence of virus. At lowest concentrations of 10 mg·l-1 ribavirin was entirely ineffective for ACLSV and 10 to 30 mg·l-1 was ineffective for PNRSV elimination. Ribavirin concentrations of 40 and 50 mg·l-1 destroyed both pathogens. However, at higher concentrations of 40 and 50 mg·l-1 ribavirin exhibited some signs of phytotoxicity on microshoots in the first sub-cultivation period. In order to test the genetic stability of the microplants after chemotherapy the amplified fragment length polymorphism (AFLP) method was applied. Plant genome stability in 'Magna Glauca' at concentrations of 40 mg·l-1 was damaged, as the presence of polymorphic AFLP markers were observed
Internet: https://content.sciendo.com/view/journals/prolas/73/3/article-p238.xml
https://hdl.handle.net/20.500.12259/99667
https://doi.org/10.2478/prolas-2019-0038
Affiliation(s): Lietuvos agrarinių ir miškų mokslų centro Sodininkystės ir daržininkystės institutas
Vytauto Didžiojo universitetas
Žemės ūkio akademija
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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