Augimo reguliatorių įtaka medlievos regeneracijai

Abstract

Research was investigated at the Laboratory of Agrobiotecnology and Institute of Biology and Plant Biotecnology at the Faculty of Agronomy in Aleksandras Stulginskis University in 2015-2016. Research in vitro was carried out with Amelanchier alnifolia Nutt. ‘Honeywood’apical meristem shoots. Isolated explants were growing on (MS) Murashige and Skoog nutrition medium without growth regulators and supplemented with different content of growth regulators: BA 0-5,0 mg l-1 and BA 0-5,0 mg l-1 and NAA 0,1 mg l-1 concentrations. Estimated microshoots formation frequency % and microshoots number per explant after four growing weeks. Amelanchier alnifolia Nutt. 'Honeywood' isolated apical shoot regeneration process was essentially driven investigate cytokinin BA and NAA auxin combinations of nutrient medium. The maximum microshoots yield, from isolated explants, obtained in nutrient medium supplimented 0,5-1,0 mg l-1 BA and 2,0-3,0 mg l-1 BA + 0,1 mg l-1 NAA. The maximum amount of microshoot from explant was obtained nutrient medium supplemented with 4.0 mg l-1 BA and 2.0 mg of BA + 0.1 mg l-1 NAA combination.