Juodųjų serbentų reversijos viruso genetinė įvairovė Lietuvoje

Abstract

Research was carried out in Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Department of Orchard Plant Genetics and Biotechnology in 2017. The aim of the research was to identify the origin of blackcurrant reversion virus in 'Gojai' cultivar using molecular methods. Samples of blackcurrant cultivar 'Gojai' showing symptoms of blackcurrant reversion disease (BRD), were collected. Blackcurrant reversion virus (BRV) was detected in all symptomatic plants. After amplification, a back part of the 3′ non-translated region of RNR2 of two new isolates of BRV were sequenced and compared with each other and with sequences available in the NCBI GeneBank. The identity between two Lithuanian isolates was 97.2 %. Further sequence analysis of BRV isolates resulted in a phylogenetic dendrogram with two branches at 96.0 % bootstrap. Significant genetic diversity among isolates were established. First branch consisted of 20.0 % isolates from Canada, New Zealand and Sweden. These isolates sequences belonged to type of BRV that causes E form of revesion disease. 80.0 % of investigated sequences were from Poland, Finland, Russia and Lithuania and they were inoculated by BRV virus, which causes R form of reversion disease.