Antioxidant activity changes during the germination of broccoli, radish and rape seeds

Abstract

Antioxidant activity was analyzed of raw and 24, 72, 120 hours germinated Brassicaceae family seeds (radish (Raphanus sativus L. var. radicula), broccoli (Brassica oleracea L. var. italica) and rape (Brassica napus L. var. oleifera)). Were detected changes in amounts of ascorbic acid, total phenols, ABTS and DPPH radical scavenging capacity during seeds germination. Seeds germination resulted in an increase of ascorbic acid content. The highest amount was in germinated rape seeds and increased by 5.1 times. Compared raw and 120 hours germinated seeds, the amounts of phenol compounds increased by 1.6 time in broccoli, whereas in the seeds of rape decreased by 0.9 times. During all germination process DPPH radical scavenging capacity increased in broccoli seeds 1.4, in radish seeds – 1.6 times. In rape seeds this index fluctuated and after 120 hours was less than in raw seeds. ABTS radical scavenging capacity was the highest in 120 hours germinated radish seeds – 2.65 mmol g-1. The statistically significant strong and medium positive correlations between the amounts of phenol compounds, ascorbic acid, DPPH and ABTS radical scavenging capacity were established in the germinated radish seeds.