Paprastojo antaninio obelyse paplitusių virusų tyrimas PGR metodu

Abstract

Antonovka apple widespread viruses was tested by PCR method. The research aims to identify viruses ACLSV, and ASGV ASPV prevalence Antonovka apple varieties in Lithuania. Studies were performed at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Plant Propagation Centre, Garden Plant Genetics and Biotechnology Laboratory. The three major apple widespread viruses tested seven different locations in Lithuania growing of all ages Antonovka variety of apple trees. For the virus detection used the PCR method. To research these viruses: ACLSV - Apple chlorotic leaf spot virus, ASGV - Apple stem grooving virus, ASPV - Apple stem pitting virus. ACLSV virus-specific 685 bp fragment and ASPV virus-specific 370 bp fragment identified in seven Antonovka variety of apple. ASGV - Apple stem grooving virus was detected in four apple trees, from 273 bp specific fragment. In three apple was found ACLSV and ASPV combination of viruses. ACLSV, ASPV, ASGV virus combination was found in four apple. These combinations like ACLSV + ASGV and ASPV + ASGV was not found. It was found that investigated different ages, in different areas of Lithuania growing seven Antonovka variety of trees are infected with one or more viruses. Most Antonovka apple varieties are widespread viruses: ACLSV - Apple chlorotic leaf spot virus and ASPV - Apple stem pitting virus