Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/60170
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biofizika / Biophysics (N011);Fizika / Physics (N002)
Author(s): Ruzgys, Paulius;Novickij, Vitalij;Novickij, Jurij;Šatkauskas, Saulius
Title: Nanosecond range electric pulse application as a non-viral gene delivery method: proof of concept
Is part of: Scientific reports [electronic resource]. London : Nature Publishing Group, 2018, vol. 8
Extent: p. 1-8
Date: 2018
Note: Article number: 15502
Keywords: Gene delivery method;Electric feld pulses;Ultrashort pulses
Abstract: Current electrotransfection protocols are well-established for decades and, as a rule, employ long micro-millisecond range electric feld pulses to facilitate DNA transfer while application of nanosecond range pulses is limited. The purpose of this paper is to show that the transfection using ultrashort pulses is possible by regulating the pulse repetition frequency. We have used 200ns pulses (10–18kV/ cm) in bursts of ten with varied repetition frequency (1Hz–1MHz). The Chinese Hamster Ovary (CHO) cells were used as a cell model. Experiments were performed using green fuorescent protein (GFP) and luciferase (LUC) coding plasmids. Transfection expression levels were evaluated using fow cytometry or luminometer. It was shown that with the increase of frequency from 100kHz to 1MHz, the transfection expression levels increased up to 17% with minimal decrease in cell viability. The LUC coding plasmid was transferred more efciently using high frequency bursts compared to single pulses of equivalent energy. The frst proof of concept for frequency-controlled nanosecond electrotransfection was shown, which can fnd application as a new non-viral gene delivery method
Internet: https://www.nature.com/articles/s41598-018-33912-y.pdf
Affiliation(s): Aplinkos tyrimų centras
Biologijos katedra
Gamtos mokslų fakultetas
Vilnius Gediminas Technical University, Lithuania
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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