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Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Stravinskaitė, Kristė;Rajackaitė, Giedrė;Janulionienė, Rasa;Jocienė, Lina;Kupčinskienė, Eugenija
Title: Evaluation of genetic structure of Impatiens Parviflora populations growing in Lithuania using several types of dominant DNA markers
Is part of: The vital nature sign [electronic resource] : 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2018, [no. 12]
Extent: p. 62-62
Date: 2018
Note: Online ISSN: 2335-8718
Keywords: Impatiens Parviflora;Small balsam;Alien species;Molecular makers
Abstract: Impatiens parviflora is an invasive annual herb which is widespread in many European countries. In Lithuania this species also is considered as successful invader of city parks and forests and is one of the most noxious alien plants. The objective of the study was to estimate genetic diversity of I. parviflora according to several types of dominant DNA markers. Twenty one populations of I. parviflora were sampled to cover all the territory of Lithuania. In total more than 300 individuals were studied, up to 16 plants were sampled in each population. The set of dominant DNA markers (4 ISSR, 8 RAPD primers and 8 AFLP primer combinations) were used for molecular analyses. Agarose gel electrophoresis and capillary electrophoresis (ABI 3130 genetic analyzer) were used for separation and identification of DNA fragments. Comparing the results of obtained data by different dominant markers, the highest polymorphism according to all used markers was assessed in the Central part of Lithuania and the least polymorphic populations were in the West part of the country. Analysis of molecular variance (AMOVA) shown that genetic diversity within populations is lower than among populations according to 3 types of DNA markers. Principal coordinate analyses (PCoA) did not reveal clear differentiation of I. parviflora into separate groups according to geographical location by ISSR and RAPD markers, however AFLP markers shown that populations from West part of Lithuania groups together and populations from East part of the country creates another group, Central Lithuanian populations were more dispersed. Comparing Bayesian clustering analyses, similar numbers of clusters were obtained by separate dominant DNA makers and results suggest that spread of I. parviflora in Lithuania might be determined by complex multiple introduction process
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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