Introduction of Geranium Robertianum L., non – volatile substances in areal part during different stages

Abstract

In XIX century research of medicinal (aromatic) plants (MAP’s) and bio-active compounds had an important role. The aim of current chemical analyses was to determin non – volatile substances in Geranium robertianum areal part. Methanolic extracts was analysed in different vegetation stages: intensive growth, flower budding, beginning of blossoming, massive blossoming and the end of blossoming. G. robertianum was introduced in 2006 in Medicinal and spice (aromatic) plants collections of Botanical Garden at Vytautas Magnus University. G. robertianum is the annual or biennial herbaceous plant of Geraniaceae family which can be found widely in Europe with the exception of the far north in temperate parts of Asia, North Africa, Atlantic area of North America. In Lithuania it grows in humid deciduous or mixed forests, bushes, ash. In this study the amount of non-volatile compounds was determined using spectrophotometry methods. The total amount of phenolic compounds was evaluated using modified Folin–Ciocalteu reagent spectrophotometry method. Modified colorimetric aluminium chloride method was carried out to determinate the total content of flavonoid compounds. Radical scavenging activity was estimated by DPPH.