Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/59413
Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Tumosaitė, Vita;Stankevičius, Mantas;Drevinskas, Tomas;Bartkuvienė, Violeta;Maruška, Audrius Sigitas
Title: Size exclusion capillary electrochromatography: development of separation method
Is part of: The vital nature sign [electronic resource] : 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2018, [no. 12]
Extent: p. 20-20
Date: 2018
Note: ONLINE ISSN: 2335-8718. Financial support from Research Council of Lithuania project 09.3.3-LMT-K 712-03-0128 is acknowledged
Keywords: Capillary electrochromatography;Agarose gel;Dextran
Abstract: Capillary electrochromatography is one of the most successful separation techniques. This separation method is a combination of the advantages of high-performance liquid chromatography and capillary electrophoresis where electric field is applied in order to create the flow of mobile phase through small diameter column [1]. This analytical technique is based on separating molecules according to mass and charge ratio and the affinity to the stationary phase. The driving force for capillary electrochromatography is electroosmosis flow, which occurs because of the liquid flow through a porous membrane when electric field is applied. In size exclusion, separation is due to molecular mass difference and uncharged molecules are driven through the column by electroosmotic flow. To our knowledge there is no report of capillary electrochromatography for size exclusion separation of the macromolecules reported yet. The aim of this research was to synthesize homogeneous monolith for the separation of macromolecules using capillary electrochromatography method with contactless conductivity detector and to optimize the separation method [3]. Agarose is a linear polysaccharide polymer that can be used in analytical methods in order to separate molecules [2]. Agarose gel was modified using epoxyamine in 2 % of NaOH medium with NaBH4 additive. The reaction was performed for 24 hours. Modified and 4 % agarose gel were mixed and washed with electrolyte. 75 μm I.D. fused silica capillary was filled with prepared modified agarose gel using pressure and was used for the analysis of 1 % dextran solutions using electrical field. Optimized conditions were: 30 mM acetic acid buffer, pH 3.35 was used as a background electrolyte, applied voltage was 4 kV, total capillary length was 45 cm, capillary length to the detector was 40 cm, separation temperature was 25 ºC, time of the analysis 60 min. The results obtained will be reported in the presentation
Internet: http://vns.microsep.org/wp-content/uploads/2018/05/AbstractbookVNS2018.pdf
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Instrumentinės analizės APC
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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