Optimisation of DNA purification using Silica Anionite as a solid phase extraction adsorbent

Abstract

Currently in science there is a growing need of pure and high-quality DNA for experiments[1]. That is why it is important for a DNA purification to be fast, efficient and relatively inexpensive. Therefore, the aim of this study was to create a stationary phase for solid phase extraction (SPE) and purification of DNA. For this work different adsorbents with attached amino groups were used as solid phase for DNA purification using different routes of synthesis of adsorbent [2] and different DNA loading buffers. Three fractions of one sample have been made – fraction of flow through when loading DNA to the column, fraction of flow through when DNA is washed with wash buffer and fraction of flow through of DNA elution. All fractions were tested using spectrometer for DNA concentration and using horizontal gel-electrophoresis to determine the quality of DNA. It has been determined, that sodium acetate buffer of pH 5.0 was the most successful purification with all adsorbent samples as it contained the least of DNA in DNA loading and DNA washing fractions and had significantly higher amount of DNA in elution fraction.