Cell viability, DNA damage and relative mitotic arrest dependence on radiation dose

Abstract

Research on in vitro X-ray irradiated cell behavior contributes significantly to the enhancement of radiotherapy treatment efficacy in clinics. The results of investigation performed with Chinese hamster ovary (CHO) cells irradiated in linear accelerator Varian Clinac DMX (X-rays, 6 MeV and 3 Gy/min) to different doses from the interval 2 - 10 Gy are presented in this paper. Irradiated cells were analyzed using COMET assay within two hours after treatment and DNA damages in cells were assessed. Also clonogenic assay was performed to determine cell viability and formed cell colony area dependence on irradiated dose. Direct dependency of cell viability, DNA damage and relative cell division capability on irradiation dose was found. It was also found that cell division arrest plateau phase starts from 6 Gy. Obtained results are discussed in the frame of radiation induced effects in irradiated cells.