Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/58814
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dc.contributor.authorBoehringer, Stephan-
dc.contributor.authorRuzgys, Paulius-
dc.contributor.authorLuca, Tamò-
dc.contributor.authorŠatkauskas, Saulius-
dc.contributor.authorGeiser, Thomas-
dc.contributor.authorGazdhar, Amiq-
dc.contributor.authorHradetzky, David-
dc.coverage.spatialGB-
dc.date.accessioned2018-10-07T01:51:51Z-
dc.date.available2018-10-07T01:51:51Z-
dc.date.issued2018-
dc.identifier.issn20452322-
dc.identifier.otherVDU02-000022345-
dc.identifier.urihttps://doi.org/10.1038/s41598-018-22280-2-
dc.descriptionFunding: Scientific Exchange Programme NMS.CH, Grant no. Sciex-NMS 14.143-
dc.description.abstractA challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanismen
dc.description.sponsorshipAplinkos tyrimų centras-
dc.description.sponsorshipBiologijos katedra-
dc.description.sponsorshipGamtos mokslų fakultetas-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 1-12-
dc.language.isoen-
dc.relation.ispartofScientific reports [electronic resource]. London : Nature Publishing Group, 2018, vol. 8-
dc.relation.isreferencedbyScopus-
dc.relation.isreferencedbyMEDLINE-
dc.relation.isreferencedbyAcademic Search Complete (EBSCO)-
dc.relation.isreferencedbyAcademic Search Premier (EBSCO)-
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)-
dc.subjectGene therapyen
dc.subjectElectrosprayen
dc.subjectTargeted gene deliveryen
dc.subject.otherBiologija / Biology (N010)-
dc.titleA new electrospray method for targeted gene deliveryen
dc.typeStraipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)-
dc.identifier.doihttps://doi.org/10.1038/s41598-018-22280-2-
dc.identifier.isiWOS:000426540800064-
dcterms.bibliographicCitation33-
dc.date.updated2019-09-03T15:51Z-
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Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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