Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/58814
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Boehringer, Stephan;Ruzgys, Paulius;Luca, Tamò;Šatkauskas, Saulius;Geiser, Thomas;Gazdhar, Amiq;Hradetzky, David
Title: A new electrospray method for targeted gene delivery
Is part of: Scientific reports [electronic resource]. London : Nature Publishing Group, 2018, vol. 8
Extent: p. 1-12
Date: 2018
Note: Funding: Scientific Exchange Programme NMS.CH, Grant no. Sciex-NMS 14.143
Keywords: Gene therapy;Electrospray;Targeted gene delivery
Abstract: A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism
Internet: https://doi.org/10.1038/s41598-018-22280-2
Affiliation(s): Aplinkos tyrimų centras
Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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