Airborne microorganisms sampling

Abstract

According to Baron and Willeke [1] an aerosol is an assembly of solid or liquid particles suspended in a gaseous medium like air. Bioaerosols contain a heterogeneous mixture of particles from animal, microbial origin and plant. In our study fungi and bacteria are main particles of bioaerosols. The size bacterial cells (0.5-40μm), fungal spores (1.0 - 40μm). The methods used in airborne sampling – filtration, impaction or liquid impingement [2]. Before calculating or identification microbes are mainly based on cultivation, microscopy, molecular biological, immunochemical or biochemical methods. Method for fungi or bacteria - cultivation methods, based on direct impaction on a growth media. After incubation, microbial colonies identified by their morphological – genus or species level. The microbes composition and concentration differ with the weather, season, location, time of the day. By cultivation of air samples from homes dominant fungi in indoor air are Penicillium, Aspergillus, Cladosporium spp.