Capillary gelelectrochomatography

Abstract

Capillary electrochromatography is an analytical technique combined with high-performance liquid chromatography and capillary electrophoresis. This analytical technique is based on separation of molecules according to mass and charge ratio and the affinity to the stationary phase. An important feature in capillary electrochromatography is the electroosmotic propulsion of the mobile phase. In our previous research, it was demonstrated, that entirely homogeneous agarose thermoreversible gel with covalently attached ionic groups and alkyl ligands can be used for reversed phase electroseparation of hydrophobic neutral molecules according to reversed phase mechanism. To our knowledge, there is no report of capillary electrochromatography for size exclusion separation of the macromolecules. Agarose is a linear polysaccharide polymer that can be used in analytical methods in order to separate molecules. The purpose of this research was to optimize the preparation and modification of agarose gel and use it for capillary gelelectrochromatography. Agarose gel was modified using epoxyamine in 2% of NaOH medium with NaBH4 additive. The reaction was performed for 24 hours. Capillary was filled with molten modified agarose gel using pressure. 75 μm I.D. fused silica capillary was filled with prepared modified agarose gel for the analysis of neutral macromolecules using electrical field. The results obtained will be reported in the presentation