Secondary metabolites from Angelica archangelica L. synergism with enilconazolum

Abstract

Secondary metabolites high structural diversity are important tools of plants needed against microbes (bacteria, fungi) and viruses. Disease resistance in plants depends on the activation of coordinated, multicomponent defence mechanisms. Bioactive plant- derived secondary metabolites are useful leads to synthesize new and more active antimicrobial agents as well as substances with new pharmacological effects by repeated structural modification. It is expected that structurally modified natural products will exhibit increased potency, selectivity, duration of action, bioavailability and reduced toxicity [1]. Trichophyton spp. are fungal species that known as tinea infections in various areas in humans and animals. Enilconazolum is used antifungal drug for the treatment of both superficial and deep infections caused by Trichophyton. Essential oils are one of the most promising groups of natural compounds for the development of safer antifungal agents. Many essential oils are only fungistatic and high concentrations are needed for fungicidal activity. To enhance the efficacy of essential oils, the combined use of different oils has been evaluated recently for synergistic effects. The combination of essential oils with synthetic antifungals will probably result in a more effective therapy. Antifungal agents were tested against fungal isolates using the agar diffusion method. We used the essential oil Angelica archangelica L. The results of the agar diffusion tests indicate that these essential oil is significantly potent against Trichophyton spp. MIC assay results exhibited strong inhibition of 7 mm in most of the experimental concentrations of 50% and 1% of agar diffusion test. The MIC synergism of the essential oil Angelica archangelica L. with enilconazolum were remarkably decreased and agar diffusion test exhibited inhibition of 20 – 25 mm sterile zone. [...]