Analysis of volatile and non-volatile biologically active compounds found in the ground segment of Silybum marianum L. during different stages of vegetation

Abstract

Analysis of volatile and non-volatile biologically active compounds found in the ground segment of Silybum marianum L. by using GC-MS system and spectrophotometric methods was completed to determine the most appropriate medicinal raw materials of the ground segment collection time and analysis of meteorological conditions for accumulation of biologically active compounds in the ground segment of Silybum marianum L. was completed to determine the optimal conditions for the accumulation of these compounds. 21 volatile compounds have been identified. The amount of total phenolic compounds of the year 2014 plant raw materials ranged from 17.80 mg / g RE (rutin equivalents) to 26.58 mg / g RE, of the year 2015 plant raw materials ranged from 23.25 mg / g RE up to 60.45 mg / g RE. Total flavonoid content of the year 2014 plant raw materials ranged from 10.86 mg / g RE up to 17.81 mg / g RE. The year 2015 plant flavonoids of the raw material content ranged from 8.02 mg / g RE up to 42.45 mg / g RE. Radical scavenging activity varied from 5.56-5.63 mg/g RE of the year 2014 and from 10.27 -11.63 mg/g RE of the year 2015. A good time to plant medicinal raw material collection is full flowering vegetation stage. The most favorable meteorological conditions during vegetation period for accumulation of biologically active compounds of milk thistle is aired conditions. Hydrothermal coefficient HTC = 0.61 and lower duration of solar lighting.