Investigation of introduction, growth tendencies and biologically active compounds accumulation in showy tick-trefoil (Desmodium Canadense (L.) DC.)

Abstract

The aim of this study - to perform phenology‘s observations of showy tick – trefoil, investigate a viability of seeds in both field and laboratory conditions, to prepare raw of pharmacy and measure biologically active compounds of showy tick – trefoil. The results of this research showed that seeds of showy tick – trefoil are better germinated in both field and laboratory, when under stratification; the viability of stratificated seeds in laboratory was 16 %, not stratificated 5 %, the viability of stratificated seeds in field was 5 %, whereas not stratificated seeds failed to germinate. It was also found that showy tick - trefoil contains many compounds, such as volatile compounds, flavonoids and phenolic compounds. In addition, this plant distinguish itself with its antioxidant activity. Using the gas chromatography - mass spectrometry method it was found that the biggest amount of votalite compounds between organs were in leaves (13,128 x 10 6 s. v), followed by the stem blossoms, then stems and, finally, seeds. Moreover, it was established that the biggest variety of volatile compounds are found and identified in the blossoms – founded 16 different compounds (identified - 11 compounds). Furthermore, the biggest amount of volatile coumpounds, between the vegetation phases, were found at the end of flowering phase (9,621 x 10 6 s. v), whereas, the biggest variety of these volatile compounds were found and identified during the intensive growth phase of the plant. Spectrophotometric analysis showed that the biggest amount of phenolic compound (16,05 RE mg/g) and flavanoid, as well as, antioxidant activity (13,8 RE mg/g) were found between the organs of showy tick-trefoil, particularly, in the parennial plant's leaves. Additionally, the biggest amount of phenolic compound between the phases of vegetation were found in butonisation (30,55 RE mg/g) and at the beggining of flowering phases (30,25 RE mg/g);