Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/56983
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dc.contributor.authorStankevičius, Mantas-
dc.contributor.authorBimbiraitė-Survilienė, Kristina-
dc.contributor.authorDrevinskas, Tomas-
dc.contributor.authorKaškonienė, Vilma-
dc.contributor.authorKornyšova, Olga-
dc.contributor.authorBartkuvienė, Violeta-
dc.contributor.authorNaujokaitytė, Gintarė-
dc.contributor.authorŠernienė, Loreta-
dc.contributor.authorMulkytė, Kristina-
dc.contributor.authorKasnauskytė, Neringa-
dc.contributor.authorMalakauskas, Mindaugas-
dc.contributor.authorMaruška, Audrius-
dc.coverage.spatialPL-
dc.date.accessioned2018-10-07T01:09:14Z-
dc.date.available2018-10-07T01:09:14Z-
dc.date.issued2016-
dc.identifier.otherVDU02-000020199-
dc.identifier.urihttps://hdl.handle.net/20.500.12259/56983-
dc.description.abstractNisin is a low molecular weight antimicrobial polypeptide or bacteriocin produced by certain strains of Lactococcus lactis subsp, lactis. It has been used as a food preservative. The earliest use of nisin in food was as a preservative in processed cheese products. The objective of this research was to study parameters for concentrating nisin from 2.5 % preparation using 100 % acetonitrile. To concentrate and clean nisin a solid phase extraction (SPE) CI8 cartridge was used. To assess nisin composition and determine the salt content in the sample after SPE purification the capillary electrophoresis with conductivity detection was applied. High-pressure liquid chromatography - electrospray ionization mass spectrometry (HPLC-ESI-MS) method was applied and optimized for qualitative and quantitative analysis of bacteriocins. Validation steps have been performed, namely repeatability, linearity, limits of detection and determination using nisin standard. This method enables determination of nisin in the biological and food samplesen
dc.description.sponsorshipBiochemijos katedra-
dc.description.sponsorshipGamtos mokslų fakultetas-
dc.description.sponsorshipInstrumentinės analizės APC-
dc.description.sponsorshipLietuvos sveikatos mokslų universitetas. Veterinarijos akademija-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 210-210-
dc.language.isoen-
dc.relation.ispartofExTech 2016, ISSS 2016: 18th international symposium on advances in extraction technologies; 22nd international symposium on separation sciences, 3-6 July, 2016, Torun, Poland : abstract book / editors B. Buszewski, J. Kowalska. Torun: Nicolaus Copernicus University, 2016-
dc.subject.otherBiologija / Biology (N010)-
dc.titleEvaluation of nisin using high performance liquid chromatography - mass spectrometryen
dc.typeKonferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)-
dcterms.bibliographicCitation0-
dc.date.updated2017-11-17T14:49Z-
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local.typeT-
item.fulltextWith Fulltext-
item.grantfulltextopen-
crisitem.author.deptInstrumentinės analizės atviros prieigos centras-
crisitem.author.deptBiochemijos katedra-
crisitem.author.deptInstrumentinės analizės atviros prieigos centras-
crisitem.author.deptBiochemijos katedra-
crisitem.author.deptBiochemijos katedra-
crisitem.author.deptBiochemijos katedra-
crisitem.author.deptGamtos mokslų fakultetas-
crisitem.author.deptŽemės ūkio akademija-
crisitem.author.deptInstrumentinės analizės atviros prieigos centras-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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