Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/56983
Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Stankevičius, Mantas;Bimbiraitė-Survilienė, Kristina;Drevinskas, Tomas;Kaškonienė, Vilma;Kornyšova, Olga;Bartkuvienė, Violeta;Naujokaitytė, Gintarė;Šernienė, Loreta;Mulkytė, Kristina;Kasnauskytė, Neringa;Malakauskas, Mindaugas;Maruška, Audrius
Title: Evaluation of nisin using high performance liquid chromatography - mass spectrometry
Is part of: ExTech 2016, ISSS 2016: 18th international symposium on advances in extraction technologies; 22nd international symposium on separation sciences, 3-6 July, 2016, Torun, Poland : abstract book / editors B. Buszewski, J. Kowalska. Torun: Nicolaus Copernicus University, 2016
Extent: p. 210-210
Date: 2016
Abstract: Nisin is a low molecular weight antimicrobial polypeptide or bacteriocin produced by certain strains of Lactococcus lactis subsp, lactis. It has been used as a food preservative. The earliest use of nisin in food was as a preservative in processed cheese products. The objective of this research was to study parameters for concentrating nisin from 2.5 % preparation using 100 % acetonitrile. To concentrate and clean nisin a solid phase extraction (SPE) CI8 cartridge was used. To assess nisin composition and determine the salt content in the sample after SPE purification the capillary electrophoresis with conductivity detection was applied. High-pressure liquid chromatography - electrospray ionization mass spectrometry (HPLC-ESI-MS) method was applied and optimized for qualitative and quantitative analysis of bacteriocins. Validation steps have been performed, namely repeatability, linearity, limits of detection and determination using nisin standard. This method enables determination of nisin in the biological and food samples
Internet: https://hdl.handle.net/20.500.12259/56983
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Instrumentinės analizės APC
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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