Analysis of antiproliferative effect of Chamerion angustifolium water extract and its fractions on several breast cancer cell lines
Author | Affiliation | |
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LT | ||
Ugenskienė, Rasa | Lietuvos sveikatos mokslų universitetas. Medicinos akademija. Onkologijos institutas | LT |
Lietuvos sveikatos mokslų universitetas. Medicinos akademija. Onkologijos institutas | LT | |
Juozaitytė, Elona | Lietuvos sveikatos mokslų universitetas. Medicinos akademija. Onkologijos institutas | LT |
Date |
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2017 |
PURPOSE: To evaluate the antiproliferative effect of the aerial part of Chamerion angustifolium (L.) Holub. (Onagraceae) extract and its fractions in vitro. This is the first study on the anti-proliferative effect of C. angustifolium on 3 distinct breast cancer cell lines. MATERIAL/METHODS: Breast cancer cell lines MCF7, MDA-MB-468 and MDA-MB-231 were exposed to different concentrations of the water extract of C. angustifolium, where DPPH radical scavenging activity was 0.018-0.443mg/ml, expressed in rutin equivalents. Cell growth was analyzed after 24, 48 and 72h of incubation. Solid-phase extraction was applied for the fractionation of C. angustifolium water extract and MDA-MB-468 cell line growth was tested using different fractions. RESULTS: The concentrations corresponding to radical scavenging activity of 0.117 and 0.266mg/ml caused MCF7 cells growth inhibition, while in the samples exposed to the highest concentration (0.355 and 0.443mg/ml) no proliferation was register, suggesting cell death. MDA-MB-468 cell analysis showed similar responses. MDA-MB-231 demonstrated cell growth inhibition following the exposure to all analyzed high extract doses (0.117-0.443mg/ml). MDA-MB-468 cells were selected to evaluate the effect of fractions. In the samples exposed to the fraction containing the highest amount (91%) of oenothein B, at the concentration of 0.117mg/ml a pronounced cell growth inhibition while at higher concentrations (0.266 and 0.443mg/ml) no cell proliferation was observed. CONCLUSIONS: The consumption of C. angustifolium herb can be advantageous, alongside with conventional breast cancer treatment.
Original research article ; authors (15) ; the Study was financially supported by the Research Council of Lithuania (grant no. MIP 084/2012 ONKOFITAS)
Journal | IF | AIF | AIF (min) | AIF (max) | Cat | AV | Year | Quartile |
---|---|---|---|---|---|---|---|---|
Advances in Medical Sciences | 2.064 | 3.035 | 3.035 | 3.035 | 1 | 0.68 | 2017 | Q3 |
Journal | IF | AIF | AIF (min) | AIF (max) | Cat | AV | Year | Quartile |
---|---|---|---|---|---|---|---|---|
Advances in Medical Sciences | 2.064 | 3.035 | 3.035 | 3.035 | 1 | 0.68 | 2017 | Q3 |
Journal | Cite Score | SNIP | SJR | Year | Quartile |
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Advances in Medical Sciences | 3.2 | 0.645 | 0.694 | 2017 | Q1 |