Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/56683
Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Burokienė, Daiva;Bagušauskaitė, Viktorija;Paužaitė, Giedrė;Malakauskiene, Asta;Filatova, Irina;Lyushkevich, Veronika;Mildažienė, Vida
Title: Microbiota of seeds and seedlings’ rhizosphere of Rhododendron smirnowii grown from seeds treated with cold plasma, vacuum and electromagnetic field
Is part of: Smart Bio: international conference, 18-20 May 2017, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2017
Extent: p. 26-26
Date: 2017
Keywords: Cold plasma;Rhododendron smirnowii;Plant stress response
ISBN: 9786098104424
Abstract: We have demonstrated recently [1] that pre-sowing treatment of Rhododendron smirnowii Trautv. seeds with electromagnetic field (EMF), vacuum and cold plasma (CP) strongly affects germination kinetics and seedling growth. Seed treatment with EMF and vacuum increased germination percentage up to 70% but CP treatments negatively affected germination. However, these methods were not estimated to have a forceful effect on microbiological cleanliness of the seeds. We succeeded to isolate 11 species of fungi, where 6 were identified as plant pathogens (Sporocadus sp., Fusarium avenaceum, Alternaria alternata, Cladosporium cladosporioides, Botrytis cinerea) and other 5 were assigned to Penicilllium genus. Differences in composition of endophytic mycobiota in seeds were statistically not significant. Long term observations revealed that seedlings grown from CP treated seeds, characterized by negative effects on germination (decreased germination percent by 23%), 13 months after sowing had considerably larger (by 69%) height, more branches and better developed roots, substantially larger (7.4-fold) total leaf surface area per plant in comparison to control plants. We have tested the hypothesis that plants grown from the treated seeds induce changes in soil microorganism community promoting plant growth. The obtained results showed that physical treatments affected the amount (colony forming units (CFU)) of rhizospheric bacteria in roots: control samples had less CFU than after treatment either with EML, CP or vacuum. 104 bacteria isolates were collected from roots and 40 isolates from soil. DNA sequencing results showed that the dominant bacterial groups were Agrobacterium sp., Bacillus sp., Burkholderia sp., Paenibacillus sp., Pseudomonas sp., Rhizobium sp
Internet: https://hdl.handle.net/20.500.12259/56683
Affiliation(s): Biochemijos katedra
Botanikos institutas
Gamtos mokslų fakultetas
Vilniaus universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

Files in This Item:
marc.xml8.44 kBXMLView/Open

MARC21 XML metadata

Show full item record
Export via OAI-PMH Interface in XML Formats
Export to Other Non-XML Formats

Page view(s)

154
checked on Mar 31, 2020

Download(s)

12
checked on Mar 31, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.