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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Medicina / Medicine (M001)
Author(s): Antanavičiūtė, Ieva;Šimatonis, Linas;Ulčinas, Orestas;Gadeikytė, Aušra;Abakevičienė, Brigita;Tamulevičius, Sigitas;Mikalayeva, Valeryia;Skeberdis, Vytenis Arvydas;Stankevičius, Edgaras;Tamulevičius, Tomas
Title: Femtosecond laser micro-machined polyimide films for cell scaffold applications
Is part of: Journal of tissue engineering and regenerative medicine. Chichester : John Wiley & Sons, 2018, vol. 12, no. 2
Extent: p. 760-773
Date: 2018
Note: 2016 Dec 11. [Epub ahead of print]
Keywords: Tissue scaffolds;Tissue engineering;methods;Cell communication;Nanotubes;Mesenchymal stromal cells;Lasers, semiconductor;methods
Abstract: Engineering of sophisticated synthetic 3D scaffolds that allow controlling behavior and location of the cells requires advanced micro/nano fabrication techniques. Ultrafast laser micro-machining employing a 1030 nm wavelength Yb:KGW femtosecond laser and a micro-fabrication workstation for micro-machining of commercially available 12.7 and 25.4 µm thickness polyimide (PI) film was applied. Mechanical properties of the fabricated scaffolds, i.e., arrays of differently spaced holes, were examined via custom-built uniaxial micro-tensile testing and finite element method simulations. We demonstrate that experimental micro-tensile testing results could be numerically simulated and explained by 2-material model, assuming that 2-6 µm width rings around the holes possessed up to 5 times higher Young's modulus and yield stress compared with the rest of the laser intacted PI film areas of "dog-bone" shaped specimens. That was attributed to material modification around the micro-machined holes in the vicinity of the position of the focused laser beam track during trepanning drilling. We demonstrate that virgin PI films provide a suitable environment for the mobility, proliferation, and intercellular communication of human bone marrow mesenchymal stem cells and discuss how cell behavior varies on the micro-machined PI films with holes of different diameters (3.1, 8.4, and 16.7 µm) and hole spacing (30, 35, 40, and 45 µm). We conclude that the holes of 3.1 µm diameter were sufficient for metabolic and genetic communication through membranous tunneling tubes between cells residing on the opposite sides of PI film but prevented the trans-migration of cells through the holes
Affiliation(s): Kauno technologijos universitetas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija. Fiziologijos ir farmakologijos institutas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija. Kardiologijos institutas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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