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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Treinys, Rimantas;Kaselis, Andrius;Jover, Emmanuel;Bagnard, Dominique;Šatkauskas, Saulius
Title: R-type Calcium channels are crucial for Semaphorin 3A– induced DRG axon growth cone collapse
Is part of: PLoS ONE [elektroninis išteklius]. San Francisco, USA : Public Library of Science, 2014, vol. 9, iss. 7
Extent: p. 1-14
Date: 2014
Keywords: Semaforinas 3A;Užpakalinių šaknelių mazgai;Kalcio kanalai;Aksonų navigacija;Neurogenezė;Augimo kūgelių kolapsas;Kalcis;Semaphorin 3A;Dorsal root ganglia;Calcium channels;Axon guidance;Neurogenesis;Growth cone collapse;Calcium
Abstract: Semaphorin 3A (Sema3A) is a secreted protein involved in axon path-finding during nervous system development. Calcium signaling plays an important role during axonal growth in response to different guidance cues; however it remains unclear whether this is also the case for Sema3A. In this study we used intracellular calcium imaging to figure out whether Sema3A induced growth cone collapse is a Ca2+ dependent process. Intracellular Ca2+ imaging results using Fura-2 AM showed Ca2+ increase in E15 mice dorsal root ganglia neurons upon Sema3A treatment. Consequently we analyzed Sema3A effect on growth cones after blocking or modifying intracellular and extracellular Ca2+ channels that are expressed in E15 mouse embryos. Our results demonstrate that Sema3A increased growth cone collapse rate is blocked by the non-selective R- and T- type Ca2+ channel blocker NiCl2 and by the selective R-type Ca2+ channel blocker SNX482. These Ca2+ channel blockers consistently decreased the Sema3A-induced intracellular Ca2+ concentration elevation. Overall, our results demonstrate that Sema3A-induced growth cone collapses are intimately related with increase in intracellular calcium concentration mediated by R-type calcium channels
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Kauno medicinos universiteto Kardiologijos institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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