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Application of electroporation method for introduction of bleomycin, DNA and photosensitizer ALFCS(4) into the cells in vitro as well as in vivo
Type of publication
Tezės Web of Science duomenų bazėje / Theses in Web of Science database (T1a1)
Title
Application of electroporation method for introduction of bleomycin, DNA and photosensitizer ALFCS(4) into the cells in vitro as well as in vivo
Is part of
Gene therapy. Great Britain : Nature Publishing Group, 2004, Vol. 11, suppl. 1
Date Issued
Date Issued |
---|
2004 |
Publisher
Great Britain : Nature Publishing Group
Publisher (trusted)
Is Referenced by
Extent
p. 156-156
Field of Science
Abstract
It is known that under a strong electric field the cell undergoes membrane permeabilization that allows exogenous molecules to enter the cell more freely. It is also known that transfer of foreign molecules strongly depends on the parameters of electric pulses. Our in vitro studies showed that photosenstizers could be much more efficiently transferred to hepatoma cells using eight 1300 V/cm, 0.1 ms pulses. Combining with photosensitization of the cell we showed that cell viability was largely suppressed in comparison with the group in which AlFcS4 transfer was not facilitated by electric pulses. Applying the method of electroporation to transfer cytotoxic drug bleomycin into Lewis Lung carcinoma in vivo, we showed that the highest suppression of tumor growth was obtained when eight 1500 V/cm, 1 ms were applied. These pulses alone neither significantly suppressed tumor growth nor induced noticeable side effects of the surrounding tissues.[...]
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Jungtinė Karalystė / United Kingdom of Great Britain and Northern Ireland (GB)