Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/50652
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Maciulevičius, Martynas;Tamošiūnas, Mindaugas;Jurkonis, Rytis;Venslauskas, Mindaugas Saulius;Šatkauskas, Saulius
Title: Analysis of metrics for molecular sonotransfer in vitro
Is part of: Molecular pharmaceutics. Washington: ACS Publications, 2015, vol. 12, iss. 10
Extent: p. 3620-3627
Date: 2015
Keywords: Sonoporacija;Doksorubicinas;Mikroburbulų nykimo greitis;Inercinės kovitacijos dozė;Sonoporation;Metric;Doxorubicin;Microbubble sonodestruction;Inertial cavitation dose
Abstract: Ultrasound induced microbubble (MB) cavitation is used to significantly enhance cell membrane permeabilization, thereby allowing delivery of various therapeutic agents into cells. In order to monitor and quantitatively control the extent of cavitation the uniform dosimetry model is needed. In present study we have simultaneously performed quantitative evaluation of three main sonoporation factors: (1) MB concentration, (2) MB cavitation extent, and (3) doxorubicin (DOX) sonotransfer into Chinese hamster ovary cells. MB concentration measurement results and passively recorded MB cavitation signals were used for MB sonodestruction rate and spectral root-mean-square (RMS) calculations, respectively. Subsequently, time to maximum value of RMS and inertial cavitation dose (ICD) quantifications were performed for every acoustic pressure value. This comprehensive research has led not only to explanation of relation of ICD and MB sonodestruction rate but also to the development of a new sonoporation metric: the inverse of time to maximum value of RMS (1/time to maximum value of RMS). ICD and MB sonodestruction rate intercorrelation and correlation with DOX sonotransfer suggest inertial cavitation to be the key mechanism for cell sonoporation. All these metrics were successfully used for doxorubicin sonotransfer prediction (R2 > 0.9, p < 0.01) and therefore shows feasibility to be applied for future dosimetric applications for ultrasound-mediated drug and gene delivery
Internet: https://hdl.handle.net/20.500.12259/50652
Affiliation(s): Biochemijos katedra
Biologijos katedra
Gamtos mokslų fakultetas
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

Files in This Item:
marc.xml10.41 kBXMLView/Open

MARC21 XML metadata

Show full item record
Export via OAI-PMH Interface in XML Formats
Export to Other Non-XML Formats

WEB OF SCIENCETM
Citations 5

4
checked on Jun 2, 2020

Page view(s)

124
checked on Oct 28, 2019

Download(s)

10
checked on Oct 28, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.