Molecular diversity of Lithuanian impatiens Glandulifera based oninter-simple sequence repeat markers

Abstract

Himalayan balsam or policeman’s helmet(Impaties glanduliferaroyle) is an annual ornamental plant with attractive flowers. Introduced to europe in the XIX century from Himalayas Moun­ tains quickly it became a garden favourite but nowadays had become a prodigious weed occu­ pying natural habitats. The objective of the research was to analyse genetic diversity of different in geography populations of I. glandulifera growing in Lithuania. four most contrasting ac­ cording to the geography I. glandulifera populations (Dieveniškės, Piktuižiai, Švėkšna, Zarasai) were selected for the investigation. Genetic diversity of I. glandulifera populations was evalu­ ated using inter­simple sequence repeat (Issr) markers. selected Issr primer generated bet­ ween 18 and 21 DNA bands each. In total, 80 DNA bands were recorded and all of them were polymorphic. Percentage of polymorphic DNA bands for separate populations of I. glandulifera ranged between 34 and 61, the mean being 43. Nei’s gene diversity interval was 0.104–0.198 and shannon’s information index ranged between 0.160–0.303. Pairwise genetic distances between populations ranged from 0.165 to 0.317. results of analyses of molecular variance showed quite similar intra and inter variation between populations (45% within and 55% among popula­ tions). The UPGMA dendrogram revealed a clear differentiation between the populations. The obtained results revealed that chosen Issr markers are valuable for the evaluation of molecular diversity of I. glandulifera populations.