Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/49414
Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Chemija / Chemistry (N003)
Author(s): Sokaitė, Gintarė;Kalvėnienė, Zenona;Kasparavičienė, Giedrė;Maruška, Audrius
Title: Extraction procedure optimization of roselle (Hibiscus Sabdariffa L.) calyces
Is part of: The vital nature sign [elektroninis išteklius] : 7-th international scientific conference : abstract book. Kaunas : Vytautas Magnus university, 2013, [no. 7]
Extent: p. 74-74
Date: 2013
Abstract: Roselle (Hibiscus Sabdariffa L.) calyces is a medicinal herbal material described in European Pharmacopoeia also known in Eastern medicine for its antihypertensive, antiinflammatory, diuretic, atherosclerosis reducing and cancer preventive effects. Pharmacological effects are addressed to polyphenolic compounds present in roselle. Aqueous extract color is intensively red; intensity represents the extent of extracted anthocyanins, mainly, cyanidin and delphinidin. The aim of this work was optimization of aqueous extraction procedure of roselle calyces. Classical and modern extraction methods were applied in order to prepare roselle extracts. Magnetic stirrer, ultrasound, microwave assisted and pressurized hot water extraction modes were used. The quality of prepared extracts was evaluated spectrophotometrically by determining coloring power, total phenolic compounds and total flavonoid content and evaluating radical scavenging activity. Total phenolic compound content was determined by Folin-Ciocalteu method. Total flavonoid content was determined by colorimetric method in the presence of aluminum chloride. Radical scavenging activity was evaluated using free DPPH• radical. Coloring power was determined by method, described in European Pharmacopoeia. Highest recovery of pigments was obtained by pressurized hot water extraction. This method also provided highest recovery of phenolic compounds (3.12 mg GAE/ml ± 1.8%) and flavonoids (0.877 mg RE/ml ± 3.73%). Highest radical scavenging activity was observed in extracts prepared with the aid of magnetic stirrer for 30 min. at 80°C temperature (0.198 mg AAE/ml ± 7.41%)
Internet: https://hdl.handle.net/20.500.12259/49414
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Lietuvos sveikatos mokslų universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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