DNA analysis of 6 Actinidia L. cultivars using SSR method

Abstract

The genus Actinidia Lindl. is large, containing between 50 and 70 species of climbing plants originating mainly in southern China [1]. Actinidia L. genus plants accumulate large quantities of biologically active substances: vitamins, phenolic compounds, various organic acids. These climbing Actinidia Lindl. genus plants are not only decorative, but the berries also accumulate a lot of ascorbic acid [2]. Until now most research is dedicated to A. deliciosa and A. chinensis species. Therefore A. kolomikta, A. arguta, A. melanandra, A. purpurea and A.callosa species and varieties genetic and chemical studies will be new not only in Lithuania, but also internationally. For genetic analysis were used young Actinidia L. leaves, which were collected in Vytautas Magnus University Kaunas Botanical Garden. The DNA was extracted of 6 cultivars young leaves with a DNA-silica columns. Amplification was carried out on a Master Gradient (Eppendorf, Germany) thermal cycler using the following temperature profile by Man et al. (2011) : 95 ° C for 5 min; then 6 cycles of 94 ° C for 50 s, 61 ° C for 50 s, and 72 ° C for 1 min; followed by 30 cycles of 94 ° C for 50 s, 56 ° C for 50 s, and 72 ° C for 1 min; finishing with 72 ° C for 8 min. The amplification products were separated on 8% denaturing polyacrylamide gel and visualized by silver staining. [...]