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The evaluation of interaction of several antioxidants by spectrophotometric methods
Date Issued |
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2015 |
Nowadays, the demand of natural antioxidants has increased, because antioxidants can reduce or even stop the progression of many chronic diseases and can restore damaged structures of the human body. Antioxidants are found in various plants and are used for production of food supplements or can be used as natural food additives. People often use different plant extracts in order to get a stronger effect, considering only biological characteristics of a single plant. However, the question do biologically active substances of plants inhibit or promote each other is not answered yet. The general purpose of this work was to evaluate the interaction between several potential antioxidants by spectrophotometric methods. Four flavonoids (kaempherol, rutin, naringenin, myricetin) and three phenolic acids (ferulic, caffeic and salicylic acids) were selected for the analysis. The total amount of phenolic compounds was evaluated by the Folin–Ciocalteu method; the total content of flavonoids was determined by aluminium trichloride assay. There were used different combinations of methanolic (75%) solutions of flavonoids or phenolic acids, including 2 or 3 components. The mixture of two components was mixed in three different ratios (1:1, 1:2, 2:1), mixture of three components in 1:1:1 ratio. The absorbance of all mixtures were compared with standard solutions absorbances.