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Type of publication: Straipsnis Clarivate Analytics Web of Science Master Journal List / Article in Clarivate Analytics Web of Science Master Journal List (S2)
Field of Science: Botanika / Botany (N013)
Author(s): Česonienė, Laima;Daubaras, Remigijus
Title: Determining of genetic diversity and genetic relationships among Lithuanian selections of Actinidia kolomikta
Is part of: Agronomy Research. Estonia : Rebellis, Vol. 5, no. 1 (2007)
Extent: p. 13-20
Date: 2007
Keywords: Aktinidija;Lapai;Augalo genetika;Actinidia kolomikta;Genome analysis;Genotypes;Genomes;Leaves;Plant genetic
Abstract: The assessment of Lithuanian cultivars and clones of A. kolomikta DNA by RAPD method corroborated the significant genetic diversity and defined the level of their relationship. Genomic DNA was isolated from fresh leaves following CTAB method. Six decamer oligonucleotide primers Akt-1, Akt-2, Akt-3, 2B, OPA-02 and OPC-02 were used for PCR and a total of 42 scorable fragments were obtained. The genetic distances fall into interval 0.059-0.914. The lowest genetic distances were calculated for the clones F2 and F4, as well for the clones F2M2 and F3M3: 0.059 and 0.097, respectively. Clones specific markers for the cultivar 'Laiba' and clone F4M4 were identified with the primers OPC-02 and 2B. The dendrogram grouped the selections by UPGMA method and revealed two main clusters at the level of 0.500 genetic distance. The cultivar 'Laiba' could be characterized as the most genetically separate accession in the A. kolomikta germplasm collection of the Kaunas Botanical Garden of Vytautas Magnus University
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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