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Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Chemija / Chemistry (N003)
Author(s): Mikalayeva, Valeryia;Daugelavičius, Rimantas
Title: Studies of multidrug efflux pump activity in salmonella enterica using nile red stain and tetraphenylphosphonium and tetraphenylarsonium cations
Is part of: The vital nature sign [elektroninis išteklius] : 5th international scientific conference, Kaunas, Lithuania, 19-21th May, 2011 : abstract book. Kaunas : Vytautas Magnus university, 2011
Extent: p. 27-27
Date: 2011
Keywords: Multidrug resistance;Pumps;Salmonella enterica
Abstract: Multidrug resistance (MDR) efflux pumps is the main reason of Salmonella enterica cell resistance to antibiotics. We used spectrofluorimetric method to assay the activity of MDR pumps. The entry of lipophilic stain, such as Nile red (NR), into the cells increases its fluorescence because of the staining on intracellular lipids. We present data on the fluorimetrical assay of accumulation of tetraphenylphosphonium (TPP+) and tetraphenylarsonium (TPAs+) ions in bacteria. Effects of RND-type MDR pump inhibitor phenylalanyl-arginyl-β-naphtylamide were also studied. In this study we demonstrated competition between NR stain and TPP+ or TPAs+ ions
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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