Use this url to cite publication: https://hdl.handle.net/20.500.12259/45393
Studies of multidrug efflux pump activity in salmonella enterica using nile red stain and tetraphenylphosphonium and tetraphenylarsonium cations
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
Studies of multidrug efflux pump activity in salmonella enterica using nile red stain and tetraphenylphosphonium and tetraphenylarsonium cations
Is part of
The vital nature sign [elektroninis išteklius] : 5th international scientific conference, Kaunas, Lithuania, 19-21th May, 2011 : abstract book. Kaunas : Vytautas Magnus university, 2011
Date Issued
| Date |
|---|
2011 |
Publisher
Kaunas : Vytautas Magnus university, 2011
Extent
p. 27-27
Abstract (en)
Multidrug resistance (MDR) efflux pumps is the main reason of Salmonella enterica cell resistance to antibiotics. We used spectrofluorimetric method to assay the activity of MDR pumps. The entry of lipophilic stain, such as Nile red (NR), into the cells increases its fluorescence because of the staining on intracellular lipids. We present data on the fluorimetrical assay of accumulation of tetraphenylphosphonium (TPP+) and tetraphenylarsonium (TPAs+) ions in bacteria. Effects of RND-type MDR pump inhibitor phenylalanyl-arginyl-β-naphtylamide were also studied. In this study we demonstrated competition between NR stain and TPP+ or TPAs+ ions.
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)
Other Identifier(s)
VDU02-000012484