Changes in myogenic stem cell respiration rate during differentiation

Abstract

Skeletal myoblast cells have high potential in cell therapy technologies, employing autonomous stem cells for the repair of the injured myocardium. We investigated the respiration rate of FR1 and FR3 cell culture, derived from rabbit skeletal muscle, during differentiation. Our results shown that differentiation decreased cellular respiration rate in both cell lines after 7 days differentiation by 54 % and 42 % in FR1 and FR3, respectively. Respiration rate of cell line FR1 characterized by higher passage number was lower by 22 % than the respiration rate of FR3. These data confirm other author’s observations with adult primate stromal cell line derived from adipose tissue that a high metabolic rate (higher oxygen consumption) together with the perinuclear arrangement of mitochondria may be valid indicators of stem cell differentiation competence, whereas departure from this profile indicate that cells are differentiating or perhaps becoming senescent.