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Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Maruška, Audrius;Stanius, Žydrūnas;Stankevičius, Mantas
Title: Methodological developments in evaluation of antioxidant activity of complex mixtures : towards miniaturization and coupling of analytical methods
Is part of: Cost B35 : “Lipid peroxidation associated disorders” work group 4 open workshop on “Natural and synthetic antioxidants”, Rzeszów, Poland 25-26 September, 2009. Rzeszów, Poland : University of Rzeszów, 2009
Extent: p. 26-26
Date: 2009
Keywords: Antioxidant activity;DPPH;Capillary electrophoresis;On-line reaction detector
Abstract: Antioxidant compounds in food play an important role as a health-protecting factor. Highly reactive free radicals and oxygen species are present in biological systems. Antioxidant compounds like phenolic acids, polyphenols and flavonoids scavenge free radicals such as peroxide, hydroperoxide or lipid peroxyl and thus inhibit the oxidative mechanisms that lead to degenerative diseases. There is a number of the free radical scavenging assays for evaluation of plant extracts. The main task of this work was to create rapid, simple and less material and time consuming method to separate analytes and evaluate antioxidant activity within a single analytical run. The on-line capillary electrophoresis-reaction (antioxidant activity) detection method can be used for a rapid evaluation of individual antioxidants in complex mixtures, particularly plant extracts. Combined UV (positive signals of all UV absorbing separated compounds) and DPPH (2,2-diphenyl-1-picrylhydrazyl) reaction detector (negative signals for colored DPPH radical bleaching) signal profiles are registered. Electropherograms of the methanolic extracts of wild hops forms cones as well as antioxidant activity detection profiles will be presented
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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