Influence of different growing regions on chemical composition of Chamerion angustifolium L

Abstract

Chamerion angustifolium L. Holub is the plant that can be used for the treatment of prostate cancer. It is believable, that phenolic compounds, especially oenothein B, with antioxidant/ radical scavenging properties are responsible for such healthful effect. C. angustifolium L. plants from six different growth sites in Lithuania were introduced in Kaunas Botanical Garden of Vytautas Magnus University. Comparison of the phytochemical composition of plants grown in their native place and after introduction was performed. Solid phase microextraction (SPME) coupled with gas chromatographic-mass spectrometric analysis was used for the evaluation of volatile compounds composition in C. angustifolium L. The total amount of phenolic compounds, flavonoids and radical scavenging activity with 1,1-diphenyl-2-picrylhydrazyl (DPPH) were determined by spectrophotometric methods. High-performance liquid chromatography (HPLC) with reaction detection was used to reveal individual bioactive compounds present in C. angustifolium L. methanolic extracts and their radical scavenging properties. SPME analysis showed the predominance of anethole, β-caryophyllene and α-humulene in the samples collected in their native places; while β-ionone, estragole, anethole and β- caryophyllene were the major compounds found in the tested samples after introduction. Interesting to note, that the total amount of phenolic compounds increased from 1.2 to 2.7 times in the plants after introduction at Kaunas Botanical Garden, while total amount of flavonoids reduced 1.2-1.8 times in 4 cases out of 6 and increased only in two plants. The radical scavenging activity remained the same in four samples and slightly increased in two introduced plants (1.1 times).[...]