Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/42356
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Kežutytė, Toma;Drevinskas, Tomas;Maruška, Audrius;Rimdeika, Rytis;Briedis, Vitalis
Title: Study of tolnaftate release from fatty acids containing ointment and penetration into human skin ex vivo
Is part of: Acta Poloniae pharmaceutica - drug research. Warsaw : Polish Pharmaceutical Society, Vol. 68, no. 6 (2011)
Extent: p. 965-973
Date: 2011
Keywords: Penetration enhancers;Fatty acids;In vitro skin penetration;In vitro release;Tolnaftate
Abstract: Five fatty acids (oleic, linoleic, myristic, lauric and capric) were incorporated in 10% (w/w) into ointment formulation and their influence on lipophilic model drug tolnaftate release in vitro and enhancing effect on tolnaftate penetration into epidermis and dermis of human skin ex vivo were investigated. The prepared ointments were tested for homogeneity, pH and rheological properties. In vitro release studies and ex vivo skin penetration experiments were carried out using Hanson and Bronaugh-type flow-through diffusion cells, respectively. Tolnaftate cumulative amount liberated from semisolids was assayed using UV-Vis spectrophotometer. After in vitro skin penetration studies, appropriately extracted human skin layers were analyzed for tolnaftate content using a validated HPLC method. Statistical analysis revealed that release rate of tolnaftate from control ointment and ointments with fatty acids was not significantly different and only 7.34ñ8.98% of drug was liberated into an acceptor medium after 6 h. Tolnaftate amount penetrating into 1 cm2 of epidermis from ointments containing oleic, linoleic, myristic and lauric acids was significantly greater (p < 0.05) than from the control ointment. Penetration enhancing ratios for these fatty acids for tolnaftate penetration into epidermis ranged from 1.48 to 1.75. In conclusion, fatty acids did not increase the liberation of tolnaftate from ointment formulation, but demonstrated their enhancing effect on tolnaftate penetration into human epidermis in vitro. Results from in vitro release experiments do not suit for prediction of the situation in the skin in vitro, if chemical penetration enhancers are incorporated into the ointment formulation
Internet: https://hdl.handle.net/20.500.12259/42356
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Lietuvos Sveikatos mokslų universiteto ligoninė
Lietuvos sveikatos mokslų universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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