Using of hesperidine for prevention poisoning of phosphororganic substances

Abstract

According to the World Health Organization, about 3 million pesticide poisonings occur worldwide each year. The use of organophosphorus substances is poorly regulated and their easy accessibility is a consequence of a large number of self-poisonings [1]. The main pathogenetic mechanism of action of organophosphorus compounds is based on the inhibition of the activity of cholinesterases - enzymes that hydrolyze acetylcholine and butyrylcholine and play an important role in the process of synaptic transmission of nerve impulse in cholinergic entities [2]. Therefore, the development of new ways of preventing poisoning with organophosphorus toxic substances is relevant. As a model substance used paraoxone. The aim of the study was to determine the activity of human serum butyrylcholinesterase in the presence of paraoxon and with the preliminary inhibition of the enzyme by the model flavonoid hesperidin. The study is based on the determination of ex vivo human serum butyrylcholinesterase activity using a modified Ellman method. This method is based on the ability of the thiocholine, the reaction product, to restore potassium hexacyanoferrate (III), which is colored yellow, to potassium hexacyanoferrate (II), which is practically unpainted. This allows direct photometric registration of the rate of enzymatic reaction.[...]