Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/100817
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Sukovas, Artūras;Šilkūnienė, Giedrė;Trumbeckaitė, Sonata;Jasukaitienė, Aldona;Degutytė-Fomins, Laima;Mildažienė, Vida;Gulbinas, Antanas;Banienė, Rasa;Dambrauskas, Žilvinas;Paškauskas, Saulius
Title: Hyperthermia potentiates cisplatin cytotoxicity and negative effects on mitochondrial functions in OVCAR-3 cells
Is part of: Journal of bioenergetics and biomembranes. New York : Springer, 2019, vol. 51, iss. 4
Extent: p. 301–310
Date: 2019
Keywords: Ovarian neoplasms;Cell line, tumor;Cisplatin;Hyperthermia, induced;Glutamate dehydrogenase
Abstract: The aim of this study was to determine the effects of hyperthermia, cisplatin and their combination on mitochondrial functions such as glutamate dehydrogenase (GDH) activity and mitochondrial respiration rates, as well as survival of cultured ovarian adenocarcinoma OVCAR-3 cells. Cells treated for 1 h with hyperthermia (40 and 43 °C) or cisplatin (IC50) or a combination of both treatments were left for recovery at 37 °C temperature for 24 h or 48 h. The obtained results revealed that 43 °C hyperthermia potentiated effects of cisplatin treatment: combinatory treatment more strongly suppressed GDH activity and expression, mitochondrial functions, and decreased survival of OVCAR-3 cells in comparison to separate single treatments. We obtained evidence that in the OVCAR-3 cell line GDH was directly activated by hyperthermia (cisplatin eliminated this effect); however, this effect was followed by GDH inhibition after 48 h recovery. A combination of 43 °C hyperthermia with cisplatin induced stronger GDH inhibition in comparison to separate treatments, and negative effects exerted on GDH activity correlated with suppression of mitochondrial respiration with glutamate + malate. Cisplatin did not induce uncoupling of oxidative phosphorylation in OVCAR-3 cells but induced impairment of the outer mitochondrial membrane in combination with 43 °C hyperthermia. Hyperthermia (43 °C) potentiated cytotoxicity of cisplatin in an OVCAR-3 cell line.[...]
Internet: https://link.springer.com/content/pdf/10.1007%2Fs10863-019-09805-8.pdf
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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